Detection of Salmonella spp., Escherichia coli, and Listeria monocytogenes in Tuna by Multiplex PCR

Fishery products are types of commodity with various levels development including the addition food additives. They very susceptible to patogenic microbial contamination hence they can cause poisoning and disease outbreaks in consumers. In this study, tuna fish spiked Salmonella spp., Escherichia coli, Listeria monocytogenes bacteria is examined for contamination. The examination these carried out using multiplex PCR (mPCR), a modern technique developed detect target DNAs simultaneously. results compared biochemical test sensitivity time-efficiency. Based on obtained, mPCR method ±1250-fold more sensitive ±80-fold time-efficient than tests. Therefore, could be recommended used as tool detection fishery products.